The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.3 [Apr-24-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr-S3_1_F16_T7.seq Fgr-S3_1_F16_T7 LENGTH:195bp ;
DIRECTION:5 ; CLONE:Fgr-S3_1_F16 ; CLONELIB: n/a
(195 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
922,381 sequences; 289,601,141 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_619172.1| (NC_003552) predicted protein [Methanosarc... 31 3.6
ref|NP_293843.1| (NC_001263) acetyl-CoA carboxylase, biotin... 31 3.6
ref|NP_598691.1| (NM_133930) expressed sequence AI843811 [M... 31 3.6
ref|NP_506694.1| (NM_074293) glutamate receptor subunit eps... 30 4.7
emb|CAB01667.2| (Z78413) predicted using Genefinder~Similar... 30 4.7
>ref|NP_619172.1| (NC_003552) predicted protein [Methanosarcina acetivorans str. C2A]
gb|AAM07652.1| (AE011148) predicted protein [Methanosarcina acetivorans str. C2A]
Length = 78
Score = 30.8 bits (68), Expect = 3.6
Identities = 15/52 (28%), Positives = 29/52 (54%)
Frame = -2
Query: 194 RAAAGQVWRNKHTYELTSKIVDKLVHGLAVPSTSRHAVEGKLC*KLKLRAHR 39
R G V+ N++ + + + ++ L+HG+ P+TS+ A G K+R H+
Sbjct: 14 RFLIGAVFSNQYFFRILNAVIFCLIHGILKPNTSKPADPG----SSKIREHK 61
>ref|NP_293843.1| (NC_001263) acetyl-CoA carboxylase, biotin carboxylase subunit
[Deinococcus radiodurans]
pir||B75558 acetyl-CoA carboxylase, biotin carboxylase subunit - Deinococcus
radiodurans (strain R1)
gb|AAF09707.1|AE001874_4 (AE001874) acetyl-CoA carboxylase, biotin carboxylase subunit
[Deinococcus radiodurans]
Length = 445
Score = 30.8 bits (68), Expect = 3.6
Identities = 19/62 (30%), Positives = 28/62 (44%), Gaps = 1/62 (1%)
Frame = +3
Query: 3 VVAAEVWHPGWGSVRSELEFLTKLTFHGMPRCAWNSESMNELINDLGG-ELVSMFVPPDL 179
+ AE HPG+G + +F HG+ ESM L + GG E+ + P +
Sbjct: 72 MTGAEAIHPGYGFMAENPDFAEMCREHGIVFIGPTPESMRALGSKAGGREIAAQSNVPTV 131
Query: 180 PG 185
PG
Sbjct: 132 PG 133
>ref|NP_598691.1| (NM_133930) expressed sequence AI843811 [Mus musculus]
gb|AAH10804.1|AAH10804 (BC010804) Unknown (protein for MGC:18896) [Mus musculus]
gb|AAH25932.1| (BC025932) expressed sequence AI843811 [Mus musculus]
Length = 420
Score = 30.8 bits (68), Expect = 3.6
Identities = 20/48 (41%), Positives = 23/48 (47%), Gaps = 2/48 (4%)
Frame = -3
Query: 145 PPRSLISSFMD--SLFQAHRGMPWKVSFVKNSSSERTEPHPGCHTSAA 8
PPR L+ S + SLF + G W S R EPHP CHT A
Sbjct: 5 PPRGLVPSLLWCLSLFLSLPGPVWLQPSPPPHPSPRAEPHP-CHTCRA 51
>ref|NP_506694.1| (NM_074293) glutamate receptor subunit epsilon [Caenorhabditis
elegans]
pir||T20939 hypothetical protein T01C3.10 - Caenorhabditis elegans
Length = 795
Score = 30.4 bits (67), Expect = 4.7
Identities = 18/58 (31%), Positives = 29/58 (49%), Gaps = 1/58 (1%)
Frame = +3
Query: 3 VVAAEVWHPGWGSVRSELEFLTKLTFHGMPRCAWNSESMN-ELINDLGGELVSMFVPP 173
V A+E + + +E FLT ++ G+P AWN+++ NDLG + PP
Sbjct: 55 VTASETYD---STTAAEQYFLTAASYTGIPIIAWNADNAGFTFENDLGPYRIIQMAPP 109
>emb|CAB01667.2| (Z78413) predicted using Genefinder~Similarity to Rat glutamate
receptor subunit epsilon (SW:NME1_RAT), contains
similarity to Pfam domain: PF00060 (Ligand-gated ion
channel), Score=201.6, E-value=4e-57, N=1~cDNA EST
yk420c10.3 comes from this gene~cDN>
emb|CAB02939.2| (Z81061) predicted using Genefinder~Similarity to Rat glutamate
receptor subunit epsilon (SW:NME1_RAT), contains
similarity to Pfam domain: PF00060 (Ligand-gated ion
channel), Score=201.6, E-value=4e-57, N=1~cDNA EST
yk420c10.3 comes from this gene~cDN>
Length = 819
Score = 30.4 bits (67), Expect = 4.7
Identities = 18/58 (31%), Positives = 29/58 (49%), Gaps = 1/58 (1%)
Frame = +3
Query: 3 VVAAEVWHPGWGSVRSELEFLTKLTFHGMPRCAWNSESMN-ELINDLGGELVSMFVPP 173
V A+E + + +E FLT ++ G+P AWN+++ NDLG + PP
Sbjct: 55 VTASETYD---STTAAEQYFLTAASYTGIPIIAWNADNAGFTFENDLGPYRIIQMAPP 109
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
Posted date: May 11, 2002 4:54 AM
Number of letters in database: 289,601,141
Number of sequences in database: 922,381
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 121,126,535
Number of Sequences: 922381
Number of extensions: 2137558
Number of successful extensions: 6268
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6268
length of database: 289,601,141
effective HSP length: 40
effective length of database: 252,705,901
effective search space used: 6064941624
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)