The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.3 [Apr-24-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr-S3_1_F21_T7.seq Fgr-S3_1_F21_T7 LENGTH:637bp ;
DIRECTION:5 ; CLONE:Fgr-S3_1_F21 ; CLONELIB: n/a
(637 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
922,381 sequences; 289,601,141 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|CAA84231.1| (Z34467) antirestriction protein [synthetic... 33 2.1
ref|NP_395405.1| (NC_003134) putative antirestriction prote... 31 8.0
>emb|CAA84231.1| (Z34467) antirestriction protein [synthetic construct]
Length = 168
Score = 33.1 bits (74), Expect = 2.1
Identities = 13/42 (30%), Positives = 23/42 (53%)
Frame = -3
Query: 377 WVESQGEADYKLRDDFFRIQSDIATIIMKEVIEEKGMFHHGP 252
W E GE DY DD +R +++ +E++E+ G+ + P
Sbjct: 96 WAEYTGECDYDAFDDAYRGEAESEEDFAREMVEDNGLLNEVP 137
>ref|NP_395405.1| (NC_003134) putative antirestriction protein [Yersinia pestis]
pir||T14723 antirestriction protein homolog - Yersinia pestis plasmid pMT1
gb|AAC13241.1| (AF053947) antirestriction protein homolog [Yersinia pestis]
gb|AAC82748.1| (AF074611) antirestriction protein [Yersinia pestis]
emb|CAB55243.1| (AL117211) putative antirestriction protein [Yersinia pestis]
Length = 168
Score = 31.2 bits (69), Expect = 8.0
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -3
Query: 377 WVESQGEADYKLRDDFFRIQSDIATIIMKEVIEEKGMFHHGP 252
W E GE DY DD +R +++ +E++++ G+ + P
Sbjct: 96 WAEYTGECDYDAFDDAYRGEAESEEDYAQEMVDDNGLLNEVP 137
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
Posted date: May 11, 2002 4:54 AM
Number of letters in database: 289,601,141
Number of sequences in database: 922,381
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 336,451,197
Number of Sequences: 922381
Number of extensions: 6891613
Number of successful extensions: 15065
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 14627
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15058
length of database: 289,601,141
effective HSP length: 115
effective length of database: 183,527,326
effective search space used: 17618623296
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)