The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.3 [Apr-24-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr-S3_1_F23_T7.seq Fgr-S3_1_F23_T7 LENGTH:382bp ;
DIRECTION:5 ; CLONE:Fgr-S3_1_F23 ; CLONELIB: n/a
(382 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
922,381 sequences; 289,601,141 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_093016.1| (XM_093016) hypothetical protein XP_093016... 35 0.19
ref|NP_011296.1| (NC_001139) Hypothetical ORF; Mmm2p [Sacch... 31 2.8
gb|AAK38266.1|AF319782_60 (AF319782) ORF57 [callitrichine h... 29 8.1
>ref|XP_093016.1| (XM_093016) hypothetical protein XP_093016 [Homo sapiens]
Length = 222
Score = 34.7 bits (78), Expect = 0.19
Identities = 23/75 (30%), Positives = 36/75 (47%), Gaps = 1/75 (1%)
Frame = +1
Query: 94 LDIEHYQNEYENGRTRGIL-RNDPLANKLGMVEDGLSYSPIKVLALTLQSCIGRTYFASD 270
L++ +N EN T GIL RN+P+ +V L+Y P + L L + Y
Sbjct: 87 LEVLEEENLAENAETMGILLRNEPMKLPSDVVTVSLAYEPPETLTGFLPYSVEGVYGVFS 146
Query: 271 LLHYFYFVTPSA*FK 315
+ F F+ P+A +K
Sbjct: 147 PPYVFTFILPAAVYK 161
>ref|NP_011296.1| (NC_001139) Hypothetical ORF; Mmm2p [Saccharomyces cerevisiae]
sp|P53083|YGW9_YEAST HYPOTHETICAL 52.0 KD PROTEIN IN NIF3-CLG1 INTERGENIC REGION
pir||S64241 hypothetical protein YGL219c - yeast (Saccharomyces cerevisiae)
emb|CAA96934.1| (Z72741) ORF YGL219c [Saccharomyces cerevisiae]
Length = 459
Score = 30.8 bits (68), Expect = 2.8
Identities = 21/52 (40%), Positives = 26/52 (49%)
Frame = -3
Query: 278 CKRSDAKYVRPIQLCKVRASTLMGE*DRPSSTMPSLLASGSFRNIPLVRPFS 123
CK S + IQ L+ E D PS TMP L+ +GSF IP+ FS
Sbjct: 74 CKISCKDAMLRIQTVIESNLLLINEQDTPSFTMPQLINNGSF-TIPITMTFS 124
>gb|AAK38266.1|AF319782_60 (AF319782) ORF57 [callitrichine herpesvirus 3]
Length = 1071
Score = 29.3 bits (64), Expect = 8.1
Identities = 21/64 (32%), Positives = 30/64 (46%)
Frame = +1
Query: 127 NGRTRGILRNDPLANKLGMVEDGLSYSPIKVLALTLQSCIGRTYFASDLLHYFYFVTPSA 306
N T I PL + + D SY+ ++ TL S G Y AS L H+ YF+ +A
Sbjct: 38 NDHTARIRALMPLEFGVFGLGDLTSYTLVREFLNTLLSLSGNGYIASILRHHVYFLLRAA 97
Query: 307 *FKH 318
F +
Sbjct: 98 SFSN 101
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
Posted date: May 11, 2002 4:54 AM
Number of letters in database: 289,601,141
Number of sequences in database: 922,381
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 210,180,340
Number of Sequences: 922381
Number of extensions: 3853424
Number of successful extensions: 8447
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 8099
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8447
length of database: 289,601,141
effective HSP length: 102
effective length of database: 195,518,279
effective search space used: 4692438696
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)