The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.1 [Apr-13-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr_contig0016.seq Fgr_contig0016
(715 letters)
Database: nr
705,144 sequences; 222,175,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_105933.1| hypothetical protein [Mesorhizobium loti] ... 38 0.084
ref|NP_012318.1| Yjl217wp [Saccharomyces cerevisiae] >gi|73... 36 0.24
gb|AAG51661.1|AC010704_5 (AC010704) hypothetical protein; 8... 33 2.7
pir||T17420 probable polyketide synthase type I - Pseudomon... 32 3.5
pir||A64918 membrane-associated protein uidC precursor - Es... 32 4.6
gb|AAG56603.1|AE005385_4 (AE005385) Z2619 gene product [Esc... 32 4.6
sp|P76655|YQIG_ECOLI HYPOTHETICAL OUTER MEMBRANE USHER PROT... 32 6.0
gb|AAB96628.1| (AF021262) daptomycin biosynthetic protein s... 32 6.0
dbj|BAA16575.1| (D90897) OUTER MEMBRANE USHER PROTEIN PMFC ... 32 6.0
>ref|NP_105933.1| hypothetical protein [Mesorhizobium loti]
dbj|BAB51719.1| (AP003006) hypothetical protein [Mesorhizobium loti]
Length = 191
Score = 37.7 bits (86), Expect = 0.084
Identities = 21/68 (30%), Positives = 36/68 (52%)
Frame = +3
Query: 141 PLKHFISATITFRTTYVHQYDQACLLLTFTKPGAPRKWIKTGIELYNEHPRYSTVTCDSW 320
P++ +A +T + Y YDQA L++ ++ WIK GIE + +S V +
Sbjct: 51 PVEGDFTAEVTVKGDYKVLYDQAGLMVRLSET----HWIKAGIEYTDGLAYFSVVVTNDT 106
Query: 321 ADWSVEAV 344
+DWS+ A+
Sbjct: 107 SDWSLVAI 114
>ref|NP_012318.1| Yjl217wp [Saccharomyces cerevisiae]
sp|P40893|YJV7_YEAST HYPOTHETICAL 22.0 KD PROTEIN IN HXT11-HXT8 INTERGENIC REGION
pir||S50710 hypothetical protein YJL217w - yeast (Saccharomyces cerevisiae)
emb|CAA83993.1| (Z34098) ORF [Saccharomyces cerevisiae]
emb|CAA89514.1| (Z49492) ORF YJL217w [Saccharomyces cerevisiae]
Length = 198
Score = 36.2 bits (82), Expect = 0.24
Identities = 19/65 (29%), Positives = 32/65 (49%)
Frame = +3
Query: 159 SATITFRTTYVHQYDQACLLLTFTKPGAPRKWIKTGIELYNEHPRYSTVTCDSWADWSVE 338
+A + + +Y YDQA +++ W+K GIE+ + H S+V + +DWS
Sbjct: 63 TAQVRVQGSYESLYDQAGIMVRIDDG----HWLKAGIEISDGHAMLSSVLTNGKSDWSTA 118
Query: 339 AVGPN 353
G N
Sbjct: 119 VYGGN 123
>gb|AAG51661.1|AC010704_5 (AC010704) hypothetical protein; 89317-90051 [Arabidopsis thaliana]
Length = 244
Score = 32.7 bits (73), Expect = 2.7
Identities = 16/52 (30%), Positives = 22/52 (41%)
Frame = +3
Query: 279 NEHPRYSTVTCDSWADWSVEAVGPNDEAGVMSGEKSVTIKAMKVEDALGVCL 434
N+ +Y V SW W E PN + + G S A + DA +CL
Sbjct: 38 NKIKKYKGVRMRSWGSWVTEIRAPNQKTRIWLGSYSTAEAAARAYDAALLCL 89
>pir||T17420 probable polyketide synthase type I - Pseudomonas fluorescens
gb|AAC38075.1| (AF081920) polyketide synthase type I; PltB [Pseudomonas
fluorescens]
Length = 2458
Score = 32.3 bits (72), Expect = 3.5
Identities = 20/50 (40%), Positives = 28/50 (56%), Gaps = 6/50 (12%)
Frame = +3
Query: 216 LLTFTKPGAPRKWIKTGIELYNEHPRYSTVT--CDS----WADWSVEAVGPNDEAG 365
+L FT GA +W+ G +L P + + CD WA WSVEA +D +G
Sbjct: 535 VLVFTGQGA--QWVGMGRDLLEREPAFLAMIRRCDQALAQWASWSVEAELRSDASG 588
>pir||A64918 membrane-associated protein uidC precursor - Escherichia coli
gb|AAC74687.1| (AE000257) membrane-associated protein [Escherichia coli K12]
Length = 417
Score = 32.0 bits (71), Expect = 4.6
Identities = 18/45 (40%), Positives = 24/45 (53%)
Frame = -3
Query: 140 SDRVRTEGGCEDVCRRRLLPDISSRLSLDGKRSFHFQLGITKKDP 6
S+ + EGG V + D+S+R LDG +SF F LG K P
Sbjct: 68 SNIIGVEGGAYYVYKLGARADMSTRWYLDGDKSFGFALGAVKIKP 112
>gb|AAG56603.1|AE005385_4 (AE005385) Z2619 gene product [Escherichia coli O157:H7 EDL933]
dbj|BAB35745.1| (AP002558) membrane-associated protein [Escherichia coli O157:H7]
Length = 342
Score = 32.0 bits (71), Expect = 4.6
Identities = 18/45 (40%), Positives = 24/45 (53%)
Frame = -3
Query: 140 SDRVRTEGGCEDVCRRRLLPDISSRLSLDGKRSFHFQLGITKKDP 6
S+ + EGG V + D+S+R LDG +SF F LG K P
Sbjct: 72 SNIIGVEGGAYYVYKLGARADMSTRWYLDGDKSFGFALGAVKIKP 116
>sp|P76655|YQIG_ECOLI HYPOTHETICAL OUTER MEMBRANE USHER PROTEIN IN RIBB-GLGS INTERGENIC
REGION PRECURSOR
pir||D65092 outer membrane usher protein pmfC precursor - Escherichia coli
(strain K-12)
gb|AAC76082.1| (AE000386) putative membrane protein [Escherichia coli K12]
Length = 821
Score = 31.6 bits (70), Expect = 6.0
Identities = 26/93 (27%), Positives = 42/93 (44%), Gaps = 7/93 (7%)
Frame = +3
Query: 81 WKQPPSTDVFTAPFRPHSIAPLKHFISATITFRTTYVHQYDQACLLLTFTKPGAPRKWIK 260
W + + + R I PLK+ +S ++TF + +Q L L + P + +
Sbjct: 506 WDASSNVNYSLSLSRDFDIGPLKN-VSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLS 564
Query: 261 TGIELYNEHPRYSTVTCDSWAD-------WSVEAVGPNDE 359
G++ ++ T SW D WSV A G NDE
Sbjct: 565 YGMQRNQDNEISHTA---SWYDSSDRNNSWSVSASGDNDE 601
>gb|AAB96628.1| (AF021262) daptomycin biosynthetic protein subunit [Streptomyces
roseosporus]
Length = 2638
Score = 31.6 bits (70), Expect = 6.0
Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 4/33 (12%)
Frame = -3
Query: 290 RVLIVQLNARLDPLSG----GAWLGEGEEKTGLIVLV 192
R V+ +RLDPL+G WL G ++ G++VLV
Sbjct: 1364 RAAAVEARSRLDPLTGRMVRAVWLDRGPDRRGVLVLV 1400
>dbj|BAA16575.1| (D90897) OUTER MEMBRANE USHER PROTEIN PMFC PRECURSOR. [Escherichia
coli]
Length = 565
Score = 31.6 bits (70), Expect = 6.0
Identities = 26/93 (27%), Positives = 42/93 (44%), Gaps = 7/93 (7%)
Frame = +3
Query: 81 WKQPPSTDVFTAPFRPHSIAPLKHFISATITFRTTYVHQYDQACLLLTFTKPGAPRKWIK 260
W + + + R I PLK+ +S ++TF + +Q L L + P + +
Sbjct: 250 WDASSNVNYSLSLSRDFDIGPLKN-VSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLS 308
Query: 261 TGIELYNEHPRYSTVTCDSWAD-------WSVEAVGPNDE 359
G++ ++ T SW D WSV A G NDE
Sbjct: 309 YGMQRNQDNEISHTA---SWYDSSDRNNSWSVSASGDNDE 345
Database: nr
Posted date: Jun 30, 2001 11:40 PM
Number of letters in database: 222,175,239
Number of sequences in database: 705,144
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 282,779,913
Number of Sequences: 705144
Number of extensions: 5866662
Number of successful extensions: 17071
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 16332
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17041
length of database: 222,175,239
effective HSP length: 115
effective length of database: 141,083,679
effective search space used: 17212208838
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)