The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.1 [Apr-13-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr_contig0018.seq Fgr_contig0018
(1122 letters)
Database: nr
705,144 sequences; 222,175,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_037439.1| non-functional folate binding protein [Hom... 60 3e-08
sp|Q26264|SM41_HEMPU 41 KD SPICULE MATRIX PROTEIN PRECURSOR... 35 1.4
pir||S12206 hypothetical protein 2 (rRNA external transcrib... 34 1.9
sp|P11994|SM50_STRPU 50 KD SPICULE MATRIX PROTEIN PRECURSOR... 34 1.9
ref|NP_116356.1| t22.3 [Tupaia herpesvirus] >gi|14210688|gb... 34 2.4
gb|AAK31278.1|AC079890_14 (AC079890) hypothetical protein [... 33 5.4
emb|CAC04021.1| (AL391398) hypothetical protein [Streptomyc... 32 7.1
>ref|NP_037439.1| non-functional folate binding protein [Homo sapiens]
gb|AAB81938.1| (AF000381) non-functional folate binding protein [Homo sapiens]
Length = 254
Score = 60.1 bits (144), Expect = 3e-08
Identities = 56/126 (44%), Positives = 64/126 (50%), Gaps = 25/126 (19%)
Frame = -3
Query: 1057 THSNYKTQKSPVSVFIVTTSPCRDWVICAPAAXXXXXXXXXXXXXGVEP*PSVTRCNHVW 878
THSNY+ K V VTTSP R+WVICAPAA EP VTR +H
Sbjct: 114 THSNYRASKESCIVIFVTTSPGREWVICAPAAFLGCGSLQAPSPES-EPSFPVTRGHHGR 172
Query: 877 PIPKHRKLIGKKFE*TI-------------------AGARPCDSRSYHESPVSPG----- 770
HRKLIG+ FE + AGARP P G
Sbjct: 173 HGDYHRKLIGQTFEWVVVRRHGGRAIGPRLSRVTKAAGARP---------PAGAGEGLRV 223
Query: 769 GHWFLI**IHPFRSRDF-*HVLALELPRLSK 680
G + I P + HVLALELP+LSK
Sbjct: 224 GFDLINAPIPPAKGVSARRHVLALELPQLSK 254
>sp|Q26264|SM41_HEMPU 41 KD SPICULE MATRIX PROTEIN PRECURSOR (HSM41) (HPSMC)
gb|AAB24285.1| (S48755) spicule matrix protein [Hemicentrotus
pulcherrimus=Japanese sea urchins, embryos, Peptide, 407
aa]
Length = 407
Score = 34.7 bits (78), Expect = 1.4
Identities = 24/67 (35%), Positives = 31/67 (45%), Gaps = 2/67 (2%)
Frame = -1
Query: 243 HPQNPTAGRPGRSPAAVTQASPVR-GQ*SRFVGVD*PLC*SAAGP-WVAKPVNQHAVEVC 70
+PQNP +G PGR+P Q PVR GQ R + GP W A V VC
Sbjct: 142 NPQNPMSGPPGRAPVMKRQNPPVRPGQGGRQIP-------QGVGPQWEAVEVTAMRAFVC 194
Query: 69 KIRYWKGAP 43
++ + P
Sbjct: 195 EVPAGRNVP 203
>pir||S12206 hypothetical protein 2 (rRNA external transcribed spacer) - mouse
Length = 153
Score = 34.3 bits (77), Expect = 1.9
Identities = 21/40 (52%), Positives = 28/40 (69%), Gaps = 1/40 (2%)
Frame = +1
Query: 544 YLVDSASSHMLVSKIK-PCMSKYKQLYSETANGSLYKLSFI 663
YLVD ASS L ++ C+S + + YSETANGSL +L F+
Sbjct: 105 YLVDPASSICLSQRLSHACLSTHGR-YSETANGSLNQLWFL 144
>sp|P11994|SM50_STRPU 50 KD SPICULE MATRIX PROTEIN PRECURSOR
pir||A27263 50K spicule matrix protein precursor - sea urchin
(Strongylocentrotus purpuratus)
gb|AAA30071.1| (M16231) spicule matrix protein SM50 precursor [Strongylocentrotus
purpuratus]
Length = 445
Score = 34.3 bits (77), Expect = 1.9
Identities = 24/67 (35%), Positives = 31/67 (45%), Gaps = 2/67 (2%)
Frame = -1
Query: 243 HPQNPTAGRPGRSPAAVTQASPVR-GQ*SRFVGVD*PLC*SAAGP-WVAKPVNQHAVEVC 70
+PQNP +G PGR+P Q PVR GQ R + GP W A V VC
Sbjct: 142 NPQNPMSGPPGRAPVMKRQNPPVRPGQGGRQIP-------QGVGPQWEAVEVTAMRAFVC 194
Query: 69 KIRYWKGAP 43
++ + P
Sbjct: 195 EVPAGRNIP 203
>ref|NP_116356.1| t22.3 [Tupaia herpesvirus]
gb|AAK57051.1| (AF281817) t22.3 [Tupaia herpesvirus]
Length = 366
Score = 33.9 bits (76), Expect = 2.4
Identities = 33/127 (25%), Positives = 53/127 (40%), Gaps = 4/127 (3%)
Frame = +2
Query: 290 SLRTPEW*KQRARSSSWGPQATPPTAGSVRRKEALGNLEGESGLPPRR----FWPCQIRC 457
S+R+ +W R W A+ + R E +GN RR FW ++
Sbjct: 248 SIRSRDW-----RCDIWYCNASDLRGVEIHRGELIGNGSQLKQAMLRRNSTRFW-VSVKI 301
Query: 458 ERRELCLATSNLNLSGASSAGGQPRHTIVTWLILPVVICLSQRLSHACLSISNYTAKLRM 637
E R+ S + SG S + R ++ W+ + +V C+ S S+ Y K R+
Sbjct: 302 ENRDRAFTVSITSGSGISH---RSRFSLSVWITMAIVFCM---FSVGFTSVGWYRLKYRV 355
Query: 638 AHYISYRLFDS 670
+ S R DS
Sbjct: 356 IKWRSVRTTDS 366
>gb|AAK31278.1|AC079890_14 (AC079890) hypothetical protein [Oryza sativa]
Length = 85
Score = 32.7 bits (73), Expect = 5.4
Identities = 19/75 (25%), Positives = 32/75 (42%), Gaps = 2/75 (2%)
Frame = +2
Query: 344 PQATPPTAGSVRRKEALGN--LEGESGLPPRRFWPCQIRCERRELCLATSNLNLSGASSA 517
P PP AGS ++ G ++ SG+ W + + +L ++NL + A
Sbjct: 5 PGRRPPAAGSAPQRNPTGGRVMDASSGISDLGLWKMDLSDDVYQLLSEVKHMNLMMETVA 64
Query: 518 GGQPRHTIVTWLILPVV 568
+ + WL LP V
Sbjct: 65 TAHSEYANMEWLPLPAV 81
>emb|CAC04021.1| (AL391398) hypothetical protein [Streptomyces coelicolor A3(2)]
Length = 225
Score = 32.3 bits (72), Expect = 7.1
Identities = 20/51 (39%), Positives = 27/51 (52%)
Frame = -3
Query: 598 CMA*SLRQAYDYWQNQPGNYRVPGLATSARSAGEI*VTGGETELAPFTADL 446
C+ L + D + PG +R PGLAT+ R +GG TELA AD+
Sbjct: 75 CVRGRLGRRRDGGERGPGGHRGPGLATARRR------SGGATELAVHCADV 119
Database: nr
Posted date: Jun 30, 2001 11:40 PM
Number of letters in database: 222,175,239
Number of sequences in database: 705,144
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 482,489,039
Number of Sequences: 705144
Number of extensions: 10620504
Number of successful extensions: 28143
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 25587
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28127
length of database: 222,175,239
effective HSP length: 120
effective length of database: 137,557,959
effective search space used: 34802163627
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)