The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.1 [Apr-13-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1605_F03_K05ZS5.seq 1605_F03_K05ZS5 0 0 0 1 638
(627 letters)
Database: nr
705,144 sequences; 222,175,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_011522.1| ATPase stabilizing factor; Stf2p [Saccharo... 42 0.003
dbj|BAA09311.1| (D50644) STF2 [Saccharomyces cerevisiae] 42 0.003
prf||1006225A ATP synthase regulating factor [Saccharomycet... 40 0.013
gb|AAF14242.1|AF110226_1 (AF110226) nuclear RNA binding pro... 38 0.066
pir||F71435 probable nuclear antigen - Arabidopsis thaliana... 37 0.15
gb|AAF14244.1|AF110228_1 (AF110228) nuclear RNA binding pro... 37 0.15
ref|NP_013431.1| Ylr327cp [Saccharomyces cerevisiae] >gi|12... 36 0.25
gb|AAB66119.1| (AF016672) F56D12.5 gene product [Caenorhabd... 35 0.33
dbj|BAA97154.1| (AB018117) gene_id:MQL5.6~pir||G71444~simil... 35 0.33
pir||G71444 hypothetical protein - Arabidopsis thaliana >gi... 33 1.2
gb|AAF14245.1|AF110229_1 (AF110229) nuclear RNA binding pro... 33 1.6
pir||T12180 probable transcription factor - fava bean >gi|2... 32 2.8
>ref|NP_011522.1| ATPase stabilizing factor; Stf2p [Saccharomyces cerevisiae]
sp|P16965|STF2_YEAST ATPASE STABILIZING FACTOR 15 KDA PROTEIN
pir||S12201 ATP synthase regulatory factor, 15K - yeast (Saccharomyces
cerevisiae)
dbj|BAA00345.1| (D00444) open reading frame [Saccharomyces cerevisiae]
emb|CAA96991.1| (Z72793) ORF YGR008c [Saccharomyces cerevisiae]
Length = 84
Score = 42.0 bits (97), Expect = 0.003
Identities = 21/60 (35%), Positives = 31/60 (51%)
Frame = +2
Query: 98 MTRSHKANDRDHKGIAEGTMLPTETVPRFFGKSGFADVDPKKVKKDGSGRGNWGTVNDDI 277
MTR++K +R+ K P++F +G P +KK GSG+GNWG D+I
Sbjct: 1 MTRTNKWTEREGKAD-----------PKYFSHTGNYGESPNHIKKQGSGKGNWGKPGDEI 49
>dbj|BAA09311.1| (D50644) STF2 [Saccharomyces cerevisiae]
Length = 65
Score = 42.0 bits (97), Expect = 0.003
Identities = 21/60 (35%), Positives = 31/60 (51%)
Frame = +2
Query: 98 MTRSHKANDRDHKGIAEGTMLPTETVPRFFGKSGFADVDPKKVKKDGSGRGNWGTVNDDI 277
MTR++K +R+ K P++F +G P +KK GSG+GNWG D+I
Sbjct: 1 MTRTNKWTEREGKAD-----------PKYFSHTGNYGESPNHIKKQGSGKGNWGKPGDEI 49
>prf||1006225A ATP synthase regulating factor [Saccharomycetales]
Length = 83
Score = 40.0 bits (92), Expect = 0.013
Identities = 20/59 (33%), Positives = 30/59 (49%)
Frame = +2
Query: 101 TRSHKANDRDHKGIAEGTMLPTETVPRFFGKSGFADVDPKKVKKDGSGRGNWGTVNDDI 277
TR++K +R+ K P++F +G P +KK GSG+GNWG D+I
Sbjct: 1 TRTNKWTEREGKAN-----------PKYFSHTGNYGQSPNHIKKQGSGKGNWGKPGDEI 48
>gb|AAF14242.1|AF110226_1 (AF110226) nuclear RNA binding protein [Nicotiana tabacum]
Length = 299
Score = 37.7 bits (86), Expect = 0.066
Identities = 15/21 (71%), Positives = 18/21 (85%)
Frame = +2
Query: 224 VKKDGSGRGNWGTVNDDIAGE 286
+K++GSGRGNWGT DDIA E
Sbjct: 107 IKREGSGRGNWGTPADDIAQE 127
>pir||F71435 probable nuclear antigen - Arabidopsis thaliana
emb|CAB10456.1| (Z97342) nuclear antigen homolog [Arabidopsis thaliana]
gb|AAF14243.1|AF110227_1 (AF110227) nuclear RNA binding protein [Arabidopsis thaliana]
emb|CAB80954.1| (AL161545) nuclear antigen homolog [Arabidopsis thaliana]
Length = 355
Score = 36.6 bits (83), Expect = 0.15
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = +2
Query: 227 KKDGSGRGNWGTVNDDIAGE 286
K+DGSGRGNWGT ++IA E
Sbjct: 164 KRDGSGRGNWGTPGEEIAAE 183
>gb|AAF14244.1|AF110228_1 (AF110228) nuclear RNA binding protein A [Spinacia oleracea]
Length = 356
Score = 36.6 bits (83), Expect = 0.15
Identities = 15/21 (71%), Positives = 18/21 (85%)
Frame = +2
Query: 224 VKKDGSGRGNWGTVNDDIAGE 286
VK++GSGRGNWGT D+IA E
Sbjct: 159 VKREGSGRGNWGTPADEIAQE 179
>ref|NP_013431.1| Ylr327cp [Saccharomyces cerevisiae]
sp|Q06177|STFL_YEAST STF2-LIKE PROTEIN YLR327C
pir||S53404 ATP synthase regulatory factor homolog YLR327c - yeast
(Saccharomyces cerevisiae)
gb|AAB64510.1| (U20618) Ylr327cp [Saccharomyces cerevisiae]
Length = 86
Score = 35.8 bits (81), Expect = 0.25
Identities = 15/42 (35%), Positives = 24/42 (56%)
Frame = +2
Query: 152 TMLPTETVPRFFGKSGFADVDPKKVKKDGSGRGNWGTVNDDI 277
T+ ++ P++F +G P VK+ G G+GNWG D+I
Sbjct: 8 TVHEAKSNPKYFTHNGNFGESPNHVKRGGYGKGNWGKPGDEI 49
>gb|AAB66119.1| (AF016672) F56D12.5 gene product [Caenorhabditis elegans]
Length = 378
Score = 35.4 bits (80), Expect = 0.33
Identities = 13/20 (65%), Positives = 16/20 (80%)
Frame = +2
Query: 227 KKDGSGRGNWGTVNDDIAGE 286
KKDG G+GNWG D++AGE
Sbjct: 196 KKDGHGKGNWGDQKDELAGE 215
>dbj|BAA97154.1| (AB018117) gene_id:MQL5.6~pir||G71444~similar to unknown protein
[Arabidopsis thaliana]
Length = 357
Score = 35.4 bits (80), Expect = 0.33
Identities = 18/47 (38%), Positives = 24/47 (50%)
Frame = +2
Query: 137 GIAEGTMLPTETVPRFFGKSGFADVDPKKVKKDGSGRGNWGTVNDDI 277
G+A G E PR + + +K++G GRGNWGT DDI
Sbjct: 135 GVANGESGDVERPPRNYDRHSRTG-HGTGMKRNGGGRGNWGTTEDDI 180
>pir||G71444 hypothetical protein - Arabidopsis thaliana
emb|CAB10532.1| (Z97343) hypothetical protein [Arabidopsis thaliana]
emb|CAB78755.1| (AL161546) hypothetical protein [Arabidopsis thaliana]
Length = 360
Score = 33.5 bits (75), Expect = 1.2
Identities = 12/17 (70%), Positives = 14/17 (81%)
Frame = +2
Query: 227 KKDGSGRGNWGTVNDDI 277
K+DG+GR NWGT DDI
Sbjct: 153 KRDGAGRANWGTTQDDI 169
>gb|AAF14245.1|AF110229_1 (AF110229) nuclear RNA binding protein B [Spinacia oleracea]
Length = 240
Score = 33.1 bits (74), Expect = 1.6
Identities = 11/20 (55%), Positives = 18/20 (90%)
Frame = +2
Query: 221 KVKKDGSGRGNWGTVNDDIA 280
+VK++GSGRGNWG ++++A
Sbjct: 44 EVKREGSGRGNWGAESEEVA 63
>pir||T12180 probable transcription factor - fava bean
emb|CAA66481.1| (X97907) transcription factor [Vicia faba]
Length = 370
Score = 32.3 bits (72), Expect = 2.8
Identities = 11/17 (64%), Positives = 15/17 (87%)
Frame = +2
Query: 227 KKDGSGRGNWGTVNDDI 277
K+DG+GRGNWGT D++
Sbjct: 174 KRDGAGRGNWGTEADEV 190
Database: nr
Posted date: Jun 30, 2001 11:40 PM
Number of letters in database: 222,175,239
Number of sequences in database: 705,144
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 238,557,781
Number of Sequences: 705144
Number of extensions: 4675688
Number of successful extensions: 395137005000
Number of sequences better than 10.0: 268721864
Number of HSP's better than 10.0 without gapping: 13078
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 13762
length of database: 222,175,239
effective HSP length: 113
effective length of database: 142,493,967
effective search space used: 13536926865
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)