The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.1 [Apr-13-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1606_F02_K04ZS5.seq 1606_F02_K04ZS5 0 0 0 1 307
(307 letters)
Database: nr
705,144 sequences; 222,175,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
pir||C72480 hypothetical protein APE2483 - Aeropyrum pernix... 34 0.22
dbj|BAB08771.1| (AB005232) gene_id:MBG8.19~pir||T04822~stro... 30 4.1
pir||T35371 probable Na+/H+ antiporter - Streptomyces coeli... 30 5.3
emb|CAB43527.1| (Y14248) Gq protein, alpha subunit [Geodia ... 29 7.0
gb|AAB84274.1| (AF030288) putative efflux protein [Brevibac... 29 7.0
gb|AAG56823.1|AE005406_2 (AE005406) orf, hypothetical prote... 29 7.0
sp|P76271|YEBS_ECOLI HYPOTHETICAL 48.3 KDA PROTEIN IN PRC-P... 29 7.0
ref|NP_108408.1| DNA mismatch repair protein MutL [Mesorhiz... 29 7.0
pir||D72588 hypothetical protein APE1177 - Aeropyrum pernix... 29 9.1
emb|CAA72331.1| (Y11592) melanogenic peroxidase [Sepia offi... 29 9.1
gb|AAC45364.1| (U56832) ORF4; similar to Vibrio harveyi rep... 29 9.1
gb|AAC60069.1| (U62919) dopamine D1A2 receptor [Anguilla an... 29 9.1
emb|CAB88464.1| (AL353815) putative integral membrane prote... 29 9.1
>pir||C72480 hypothetical protein APE2483 - Aeropyrum pernix (strain K1)
dbj|BAA81499.1| (AP000064) 186aa long hypothetical protein [Aeropyrum pernix]
Length = 186
Score = 34.3 bits (77), Expect = 0.22
Identities = 22/59 (37%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
Frame = +3
Query: 6 PRVRNQLLQQTPTSTHLPQLPNITMSDYKPTEHDGLRKD--GQPDGRVGTGEFAHGKVDP 179
P V N Q S L + P+I + + P+ H G D G+P G VG GE H P
Sbjct: 40 PVVFNLSPQSCGNSVQLIRCPHINPTHFPPSWHCGKVYDARGEPCGEVGGGEEGHEPPQP 99
Query: 180 H 182
H
Sbjct: 100 H 100
>dbj|BAB08771.1| (AB005232) gene_id:MBG8.19~pir||T04822~strong similarity to unknown
protein [Arabidopsis thaliana]
Length = 540
Score = 30.0 bits (66), Expect = 4.1
Identities = 13/40 (32%), Positives = 20/40 (49%)
Frame = +3
Query: 12 VRNQLLQQTPTSTHLPQLPNITMSDYKPTEHDGLRKDGQP 131
+ L+Q P ++HLP P T ++ +H L GQP
Sbjct: 450 ISQDLVQGAPHNSHLPPRPLFTPQQFQYPKHQSLIATGQP 489
>pir||T35371 probable Na+/H+ antiporter - Streptomyces coelicolor
emb|CAB45470.1| (AL079348) putative Na(+)/H(+) antiporter [Streptomyces coelicolor
A3(2)]
Length = 514
Score = 29.6 bits (65), Expect = 5.3
Identities = 24/75 (32%), Positives = 38/75 (50%), Gaps = 12/75 (16%)
Frame = -3
Query: 284 IVLSELVIAAVVAGALETSTATAARGLSALDTSFMGVNLAVSELASADA----------- 138
+ LSEL + ++ G + ++ AAR + + + L V +A D
Sbjct: 1 MTLSELYVMLLIGGTVLLASIAAARAAHRIGLPSLLLFLCVGLVAGEDGLGLRFDDAQLA 60
Query: 137 -AVGLAILAETVVLSGLVVRHGDVWEL 60
A+G A LA +V GL R DV +L
Sbjct: 61 QALGAAALALILVEGGLTTRWSDVRKL 87
>emb|CAB43527.1| (Y14248) Gq protein, alpha subunit [Geodia cydonium]
Length = 355
Score = 29.3 bits (64), Expect = 7.0
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +3
Query: 15 RNQLLQQTPTSTHLPQLPNITMSDYKPTEHDGLR 116
RN+ T +L LP I+ +DY PT D LR
Sbjct: 142 RNEYQLSDSTKYYLDDLPRISSNDYVPTTQDVLR 175
>gb|AAB84274.1| (AF030288) putative efflux protein [Brevibacterium linens]
Length = 489
Score = 29.3 bits (64), Expect = 7.0
Identities = 29/73 (39%), Positives = 36/73 (48%)
Frame = -3
Query: 305 LSILVYAIVLSELVIAAVVAGALETSTATAARGLSALDTSFMGVNLAVSELASADAAVGL 126
+SIL+ VLS + AAV+AG +TA AA A F + A AA+GL
Sbjct: 96 ISILLVGRVLSGMGAAAVMAGG--AATAAAAFNGPARSRMFAAIGTA--------AAIGL 145
Query: 125 AILAETVVLSGLV 87
A A VV GLV
Sbjct: 146 A--AGPVVSGGLV 156
>gb|AAG56823.1|AE005406_2 (AE005406) orf, hypothetical protein [Escherichia coli O157:H7
EDL933]
Length = 427
Score = 29.3 bits (64), Expect = 7.0
Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
Frame = -3
Query: 197 LDTSFMGVNLA---VSELASADAAVGLAILAETVVLSGLVVRHGDVWELW 57
LD +G+ +A V + A A VGL V+L+ + + H +V ELW
Sbjct: 165 LDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELW 214
>sp|P76271|YEBS_ECOLI HYPOTHETICAL 48.3 KDA PROTEIN IN PRC-PPHA INTERGENIC REGION
pir||A64945 probable membrane protein b1833 - Escherichia coli
dbj|BAA15641.1| (D90826) Paraquat-inducible protein A. [Escherichia coli]
dbj|BAA15646.1| (D90827) Paraquat-inducible protein A. [Escherichia coli]
gb|AAC74903.1| (AE000277) orf, hypothetical protein [Escherichia coli K12]
dbj|BAB35966.1| (AP002558) hypothetical protein [Escherichia coli O157:H7]
Length = 427
Score = 29.3 bits (64), Expect = 7.0
Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
Frame = -3
Query: 197 LDTSFMGVNLA---VSELASADAAVGLAILAETVVLSGLVVRHGDVWELW 57
LD +G+ +A V + A A VGL V+L+ + + H +V ELW
Sbjct: 165 LDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELW 214
>ref|NP_108408.1| DNA mismatch repair protein MutL [Mesorhizobium loti]
dbj|BAB53869.1| (AP003013) DNA mismatch repair protein; MutL [Mesorhizobium loti]
Length = 627
Score = 29.3 bits (64), Expect = 7.0
Identities = 22/61 (36%), Positives = 30/61 (49%)
Frame = -3
Query: 287 AIVLSELVIAAVVAGALETSTATAARGLSALDTSFMGVNLAVSELASADAAVGLAILAET 108
A V+ ELV A+ AGA TA GL+ + + G + ELA A A + LAE
Sbjct: 24 ASVVKELVENALDAGASRVEVVTAGGGLNLIRVTDDGSGIPEPELALAIARHCTSKLAED 83
Query: 107 V 105
+
Sbjct: 84 I 84
>pir||D72588 hypothetical protein APE1177 - Aeropyrum pernix (strain K1)
dbj|BAA80162.1| (AP000061) 411aa long hypothetical protein [Aeropyrum pernix]
Length = 411
Score = 28.9 bits (63), Expect = 9.1
Identities = 22/73 (30%), Positives = 36/73 (49%), Gaps = 5/73 (6%)
Frame = -3
Query: 293 VYAIVLSELVIAA-----VVAGALETSTATAARGLSALDTSFMGVNLAVSELASADAAVG 129
VYA + + IA+ V+ G + A+ L ++ F+GV LA + A +G
Sbjct: 58 VYAALFAASTIASAALNGVLLGFMMFRLQFASIALGSIAKLFVGVGLAAIGYKALAAVIG 117
Query: 128 LAILAETVVLSGLVVRHG 75
L + T+V+ G V G
Sbjct: 118 LLTMPLTIVIVGTTVLLG 135
>emb|CAA72331.1| (Y11592) melanogenic peroxidase [Sepia officinalis]
Length = 926
Score = 28.9 bits (63), Expect = 9.1
Identities = 22/66 (33%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
Frame = -3
Query: 287 AIVLSELVIAAVVAGALETSTATAARGLSALDTSFMGVNLAVSELASADAA-VGLAILAE 111
A V+ AA VAG + A AAR A DT+ A + A+A AA A +AE
Sbjct: 102 AAVVGAARAAAGVAGNAAAAAAAAARAAGATDTAVAAAAAAANAAAAAVAAGADAATIAE 161
Query: 110 TVVLSGL 90
+ +
Sbjct: 162 AAAAAAI 168
>gb|AAC45364.1| (U56832) ORF4; similar to Vibrio harveyi repressor protein encoded
by GenBank Accession Number L26221 [Aeromonas
hydrophila]
Length = 283
Score = 28.9 bits (63), Expect = 9.1
Identities = 15/30 (50%), Positives = 17/30 (56%), Gaps = 4/30 (13%)
Frame = -1
Query: 187 ASWGSTLP*ANSPVPTRPSGWPS----LRRPSCS 98
A G P +P PT PS WPS LR P C+
Sbjct: 245 ARHGGPTPITAAPCPTPPSTWPSSSPTLRSPRCA 278
>gb|AAC60069.1| (U62919) dopamine D1A2 receptor [Anguilla anguilla]
Length = 445
Score = 28.9 bits (63), Expect = 9.1
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = -3
Query: 278 LSELVIAAVVAGALETSTATAARGLSALDTSFMGVNLAVSELASA 144
LS L+++ ++ L + T R L + T+F ++LAVS+L A
Sbjct: 28 LSSLIVSTLLGNTLVCAAVTKFRHLRSKVTNFFVISLAVSDLLVA 72
>emb|CAB88464.1| (AL353815) putative integral membrane protein [Streptomyces
coelicolor A3(2)]
Length = 333
Score = 28.9 bits (63), Expect = 9.1
Identities = 23/61 (37%), Positives = 33/61 (53%), Gaps = 4/61 (6%)
Frame = -3
Query: 269 LVIAAVVA--GALETSTATA--ARGLSALDTSFMGVNLAVSELASADAAVGLAILAETVV 102
L ++AV+A G L STAT A ++ L T MG+ L + AA +A+ V+
Sbjct: 43 LTLSAVIAACGILTDSTATVIGAMIIAPLSTPIMGIALGAVQRRRTTAAAFVALGCLLVI 102
Query: 101 LSGLV 87
L G V
Sbjct: 103 LVGAV 107
Database: nr
Posted date: Jun 30, 2001 11:40 PM
Number of letters in database: 222,175,239
Number of sequences in database: 705,144
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,903,689
Number of Sequences: 705144
Number of extensions: 1488732
Number of successful extensions: 4871
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 4790
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4866
length of database: 222,175,239
effective HSP length: 77
effective length of database: 167,879,151
effective search space used: 4029099624
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)